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人G-CSF ELISA試劑盒原裝Abbkine說(shuō)明書(shū)北京代理
最近更新時(shí)間：2018-3-19
提 供 商：北京冬歌博業(yè)生物科技有限公司資料大小：530.8KB
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- Product name
EliKine™ Human G-CSF ELISA Kit
- Reactivity
Human
- Applications
ELISA
- Application notes
EliKine™ Human G-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate G-CSF in samples. An antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G-CSF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
- Detection method
Colorimetric
- Sample type
Cell culture supernatants, Other biological fluids, Plasma, Serum
- Assay type
Sandwich ELISA (quantitative)
- Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Fig.1. Human G-CSF Standard Curve.
Fig.2. EliKine™ Human G-CSF ELISA Kit is a sandwich ELISA to quantitate GCSF in samples.
Product Properties
- Kit components
• Human G-CSF microplate
• Human G-CSF standard
• Human G-CSF detect antibody
• Avidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers. - Features & Benefits
EliKine™ Human G-CSF ELISA Kit has high sensitivity and excellent specificity for detection of Human G-CSF. No significant cross-reactivity or interference between Human G-CSF and analogues was observed.
- Calibration range
31.25 pg/mL-2000 pg/mL
- Limit of detection
16 pg/mL
- Usage notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate. - Storage instructions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
- Shipping
Gel pack with blue ice.
- Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Additional Information
- Background
The protein encoded by G-CSF gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for G-CSF gene.
- Gene ID
1440
- Alternative names
C17orf33; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2
- Accession
P09919