Anti-Wheat Germ Agglutinin antibody （FITC）說明書
From:ABCAM
Catalog Number : RS-20528R
Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water. ）

Background: WGA is a N acetylglucosamine specific lectin and is synthesized in developing and germinating embryos, roots and meristematic part of the leaves. Lectin interaction with certain carbohydrates are as specific as enzyme to substrate or antigen to antibody interactions and have been used to study protein glycosylation. WGA has been reported to have a role in dormancy and plant defence, but its characteristic biochemical properties and its predominance in growing and meristematic tissues, and its osmotic- and drought-stress inducibility suggest that this protein might be involved in a much wider range of functions.

Specificity : •Anti-WGA is a horse monoclonal antibody, FITC conjugated
           • specific forWGA of mo
• Protein G affinity chromatography purification, purity :>95%
• Isotype: IgG 

Application : • FCM:  1:20-100
            • Immunohistochemistry : 1:20-1:200
            • Immunocytochemistry: 1:20-1:200
• Excitation spectrum: 490-495nm
• Emission spectrum: 520-530nm
• Optimal working dilutions must be determined by the end user.
• protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-Wheat Germ Agglutinin antibody （