Anti-HA tag/FITC抗體說(shuō)明書(shū)  

Catalog Number :RsF-0966R  

Quantity size : 0.2ml （2mg/ml; Reconstitute with 0.5 ml of sterile distilled water.）

Background:

Human influenza hemagglutinin （HA） is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Specificity :

· anti- HA Tag  is a rabbit polyclonal antibody, FITC conjugated

· specific for HA Tag of human, mouse and rat

· epitope mapping near the amino terminus of HA Tag of human,

mouse rat, pig origin

· human, mouse rat pig cross reacted

· affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 25kDa

Application :

· FCM: 1:20-100

· Immunohistochemistry : 1:20-200

· Immunocytochemistry: 1:20-200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

· Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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