Rabbit Anti-IL6抗體說明書

FROM：Abcam

Catalog Number : ab6672   

Quantity size :   200ul  （dilute with pH 7.4  0.01 M PBS or antibody diluent ）

Background:

 The antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays （ie. IL-1 stimulated IL-6 production from fibroblasts）. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule （21-28 kDa）. The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG （heat inactivated） is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

Specificity :        · anti- IL6 is a Rabbit polyclonal antibody unconjugated

 · specific for IL6 of Mouse, Rat, Human, Pig

·use for western blotting,elisa, immunoprecipitation   and immunohistochemistry

·  Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

·  mol wt: 23kDa

Application:       · Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

·  Optimal working dilutions must be determined by the end user.

Storage:     Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.

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