人抗心磷脂抗體IgA(ACA-IgA)ELISA試劑盒
Generic Name：Human ACA-IgA ELISA Kit
實(shí)驗(yàn)原理：
本試劑盒用于測(cè)定人血清，血漿及相關(guān)液體樣本中抗心磷脂抗體IgA（ACA-IgA）的含量。應(yīng)用雙抗原夾心法測(cè)定標(biāo)本中人抗心磷脂抗體IgA（ACA-IgA）水平。用純化的抗心磷脂抗體IgA（ACA-IgA）抗原包被微孔板，制成固相抗原，往包被抗原的微孔中依次加入ACA-IgA，再與HRP標(biāo)記的抗心磷脂抗體IgA（ACA-IgA）抗原結(jié)合，形成抗原-抗體-酶標(biāo)抗原復(fù)合物，經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的抗心磷脂抗體IgA（ACA-IgA）抗體呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中人抗心磷脂抗體IgA（ACA-IgA）濃度。
Principle of the assay
The kit assay Human ACA-IgA level in the sample，use Purified Human ACA-IgA antigen to coat microtiter plate wells, make solid-phase antigen, then add ACA-IgA to wells, Combined ACA-IgA antigen which With HRP labeled ,become antigen –antibody - enzyme- antigen complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACA-IgA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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