大鼠促卵泡激素（FSH）酶聯(lián)免疫分析（ELISA）試劑盒
實(shí)驗(yàn)原理：本試劑盒本試劑盒僅供研究使用，用于測定大鼠血清，血漿及相關(guān)液體樣本中促卵泡激素（FSH）的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中大鼠促卵泡激素（FSH）水平。用純化的大鼠促卵泡激素（FSH）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入促卵泡激素（FSH），再與HRP標(biāo)記的FSH抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的大鼠促卵泡激素（FSH）呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中大鼠促卵泡激素（FSH）濃度。
Drug Names
Generic Name：Rat Follicle-stimulating hormone (FSH) ELISA Kit.
Purpose
This kit allows for the determination of FSH concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat FSH level in the sample，use Purified Rat FSH antibody to coat microtiter plate wells, make solid-phase antibody, then add FSH to wells, Combined FSH which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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