小鼠5羥色胺（5HT）酶聯(lián)免疫分析(ELISA)試劑盒
實驗?zāi)康?/strong>：本試劑盒僅供研究使用，用于測定小鼠血清，血漿及相關(guān)液體樣本中5羥色胺（5HT）的含量。
實驗原理：本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中小鼠5羥色胺（5HT）水平。用純化的小鼠5羥色胺（5HT）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入小鼠5羥色胺（5HT）再與HRP標(biāo)記的5羥色胺（5HT）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的5羥色胺（5HT）呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中小鼠5羥色胺（5HT）濃度。
Drug Names
Generic Name：Mouse 5-Hydroxytryptamine(5HT) ELISA Kit.
Purpose
This kit allows for the determination of 5HT concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Mouse 5HT level in the sample，use Purified Mouse 5HT to coat microtiter plate wells, make solid-phase antibody, then add 5HT to wells, Combined 5HT antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5HT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
