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pRNAT-U6.2/Lenti

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產(chǎn)品型號(hào)SD1259

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更新時(shí)間：2017-07-21 14:49:44瀏覽次數(shù)：533次

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pRNAT-U6.2/Lenti siRNA expression vector is compatible with the Invitrogen ViraPowerTM Lentiviral_Expression System. It is designed for siRNA expression in both dividing and nondividing cells.

詳細(xì)介紹

Description: pRNAT-U6.2/Lenti siRNA expression vector is compatible with the Invitrogen ViraPowerTM Lentiviral_Expression System. It is designed for siRNA expression in both dividing and nondividing cells. The siRNA insert can be conveniently cloned between the BamH I and Xho I sites under the control of the U6 promoter. The vector contains a neomycin resistance gene for establishing stable cell lines and a coral GFP gene for tracking transfection efficiency. Both of these are driven by CM and SV40 promoters, respectively. The vector uses HIV 3'LTR for viral transcription and a Rous sarcoma enhancer/promoter joined to HIV 5'LTR （PRSV/5'LTR） for packaging.

 
Download Protocol:tm0175
Forward Sequencing Primer:
DA0011:pRNA-U6 Forward （TACGATACAAGGCTGTTAGAGAG）
Reverse Sequencing Primer:
DA0012:pRNA Reverse （TAGAAGGCACAGTCGAGG）
Detailed Map:
 
5' LTR:1-410
3' LTR:4906-5140
ColE1 ori:7220-8106
Polylinker:2200-2275
U6 Promoter:1873-2199
CMV Promoter:2320-2907
cGFP:2924-3643
SV40:3651-3996
Neomycin:4037-4831
Ampicillin:6300-7160


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