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上海研盟生物科技有限公司
上海研盟生物科技有限公司
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“Anti-AT2R抗體,血管緊張素Ⅱ受體1抗體"抗體規(guī)格0.1ml、0.2ml、1ml，抗體產品有效期一年，質保三個月，質保期內出現任何質量問題或是出不了實驗結果均可免費退換：
上海研盟生物科技有限公司Anti-AT2R抗體,血管緊張素Ⅱ受體1抗體*，主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取?？头?/p>
簡單介紹：
中文名稱：血管緊張素Ⅱ受體1抗體
英文名稱：Anti-AT2R
產品編號：byk-0438R
產品別名：AG2S; Agtr 1; Agtr1; AGTR1_HUMAN; Agtr1a; AGTR1B; Ang II; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; Angiotensin II type-1 receptor; Angiotensin receptor 1; Angiotensin receptor 1B; AT 1B; AT 1r; AT-1B; AT-1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; Type 1B angiotensin II receptor; Type-1 angiotensin II receptor.
產品規(guī)格：0.1ml、0.2ml、1ml
產品用途：科研實驗
產品價格：請報價
說明書：請?zhí)砑涌头苯铀魅?/p>
抗體來源：該指標有兩種產品，一種是兔來源抗體，一種是鼠來源抗體
克隆類型：兔來源為多克隆抗體，鼠來源單克隆抗體
交叉反應：請索取說明書查看
性 狀：Lyophilized or Liquid
濃 度：1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-AT2R抗體,血管緊張素Ⅱ受體1抗體產品介紹：Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLC?is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites.
合適的抗體稀釋度：
抗體的濃度是免疫染色的關鍵，如果抗體濃度過高，抗體分子過多于抗原決定簇，可導致抗體結合減少，產生陰性結果。此陰性結果并不一定是缺少抗原，而是由于抗體過量，這種現象類似于凝集反應中的前帶效應（prozone effect）。因此，必須使用一系列稀釋做“棋盤式效價滴定”，檢測抗體的合適稀釋度，以得到zui大強度的特異性染色和zui弱的背景染色?？贵w稀釋度應根據：1.抗體效價高，溶液中特異性抗體濃度越高，工作稀釋度越高；2.一般講，應用的抗體稀釋度越大，溫育時間越長；3.對于抗體與非特異性蛋白的結合，只有高稀釋度時才能防止其非特異性背景染色；4.稀釋用緩沖液的種類，標本的固定和處理過程等也可影響稀釋度，所以合適的稀釋度應根據具體情況測定?？贵w的稀釋主要是指*抗體，因為*抗體中特異性抗體合適的濃度是關鍵，應用高稀釋度*抗體僅高親和力的特異性染色反應，減少或消除其中交叉抗體反應。
抗原修復方法：
方法1：沸水浴修復，將盛有修復液和玻片的燒杯置于沸水浴環(huán)境，保持外部沸騰狀態(tài)15min，自然冷卻至室溫。
方法2：微波修復，將盛有修復液和玻片的燒杯置于微波爐中，高火5min，停火3min，中火5min，自然冷卻至室溫。
方法3：高壓修復，修復液加入高壓鍋加熱至沸騰，放入玻片，封蓋加壓持續(xù)加熱至噴氣時開始計時修復2min，自然冷卻至室溫。
方法4：使用*修復，將*修復液加入到組織上，37℃，消化30min
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