熒光素標記的鏈霉親和素,FITC-Streptavidin

【資料簡介】

英文名稱：FITC-Streptavidin,Fluorescein-Streptavidin

中 文 名：熒光素標記鏈霉親和素，FITC標記的鏈霉親和素。

Excitation Laser：Blue Laser (488 nm)

一種綠色熒光標記的鏈霉親和素，吸收波長為488nM，發(fā)射波長為510nM。

Description：

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

應(yīng)用簡介：

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

基本數(shù)據(jù)：

Molecular Weight ～60 kDa (tetramer)

Concentration 5 mg/ml

Modification level 2.8 FITC/tetramer

 

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鏈霉親和素其他相關(guān)產(chǎn)品：

Streptavidin-FITC

Streptavidin-TRITC

Streptavidin-CY5

Streptavidin-CY3

Streptavidin-thiol

FITC-BSA

TRITC-BSA

BAS-CY5

BAS-CY3

BAS-Biotin

Concanavalin A-CY5

Concanavalin A-CY3

Concanavalin A-CY5.5

Concanavalin A-FITC

Concanavalin A-Biotin

Concanavalin A-SH

Concanavalin A-TRITC

 

鏈霉親和素(SA)簡述：

鏈霉親和素(SA)是Streptomyces avidinii菌的分泌物，其分子量及結(jié)合生物素的能力與雞蛋清中的親和素相似，等電點6.0，非特異性結(jié)合遠比親和素低。

 鏈霉親和素(SA)的來源：

SA 是Streptomyces avicllrdi菌培養(yǎng)過程中的分泌產(chǎn)物，主要通過2一亞氨基生物索親和層析法提純，1L培養(yǎng)液中含蛋白10~60mg。鏈霉親和素是四聚體蛋白，大小為66KDa。一分子鏈霉親和素可以高度特異性地與四分子生物素結(jié)合，兩者之間的親和力極為強烈。

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.