anti-phospho-Akt（Thr308）抗體說(shuō)明書

【資料簡(jiǎn)介】

anti-phospho-Akt（Thr308）抗體說(shuō)明書

Catalog Number:Rs-2720R

Quantity size:0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）

 

Background: AKT, also known as protein kinase B （PKB）, is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 （PKB alpha）, AKT2 （PKB beta） and AKT3 （PKB gamma） with AKT2 and AKT3 being approximay 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology （PH）domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase （a lipid kinase activated by growth factors, cytokines and insulin）. PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

anti-phospho-Akt（Thr308）抗體說(shuō)明書

 

Specificity :  ·anti-Akt is a rabbit monoclonal antibody unconjugated

·specific for Akt of human, mouse, rat

·use for western blot, elisa, immunoprecipitation

and immunohistochemistry

·affinity chromatography purification, purity :>95%

·isotype: IgG

·mol wt: 91kDa

 

Application :· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

· Western blotting 1:100-500

· Optimal working dilutions must be determined by the end user.

 

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

anti-phospho-Akt（Thr308）抗體說(shuō)明書