Anti-Rad51抗體,Rad51抗體抗體溶解方法及分裝：
1、購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、以規(guī)格為 0.1ml 的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加 0.1ml 雙蒸水到試管內(nèi)，混勻，-20℃保存，若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格*的（如：0.2ml）雙蒸水溶解，
混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存，若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加
標簽上規(guī)格的 50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
抗體溶解后，將其分裝成 5-10 支（以 0.1ml 抗體為例，20ulx5 支或 10ulx10 支）， -20℃保存，用
一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度 1：100、1：200、1：400 稀釋，如果背景高，還可再稀釋，選一個zui
佳的稀釋比例，再正式做，效果。
產(chǎn)品名稱:Anti-Rad51抗體,Rad51抗體
產(chǎn)品編號：BYK-10145R
英文名稱：Anti-Rad51
中文名稱：Rad51抗體
英文別名：BRCA1/BRCA2 containing complex, subunit 5; BRCC 5; BRCC5; DNA repair protein RAD51 homolog 1; DNA repair protein rhp51; E coli RecA homolog; HGNC:9817; Homolog of E coli RecA; homolog of S cerevisiae RAD51; HRAD51; HsRad51; HsT16930; Rad 51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog; RAD51 homolog S. cerevisiae; RAD51 S cerevisiae homolog; RAD51A; RECA; RecA homolog E. coli; RecA like protein; RecA, E. coli, homolog of; recombination protein A.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Rad51抗體,Rad51抗體
英文介紹：The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.
此產(chǎn)品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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