人Kruppel樣因子2(KLF2)elisa測定北京方程生物免費(fèi)代測：

樣品如果不立即分析，應(yīng)分裝后冷凍保存，且避免反復(fù)凍融。

血清——用干凈試管收集血液，室溫凝固2小時或4℃過夜，離心1000×g 10分鐘，收集血清。立即分析或分裝后-20℃冷凍保存。

細(xì)胞培養(yǎng)、組織勻漿、體液——離心去除沉淀，立即分析或分裝后-20℃冷凍保存。

人Kruppel樣因子2(KLF2)elisa測定 加樣在ELISA中一般有3次加樣步聚，即加標(biāo)本，加酶結(jié)合物，加底物。加樣時應(yīng)將所加物加在LEISA板孔的底部，避免加在孔壁上部，并注意不可濺出，不可產(chǎn)生氣泡。加標(biāo)本一般用微量加樣器，按規(guī)定的量加入板孔中。每次加標(biāo)本應(yīng)更換吸嘴，以免發(fā)生交叉污染，也可用一次性的定量塑料管加樣。有此測定（如間接法ELISA）需用稀釋的血清，可在試管中按規(guī)定的稀釋度稀釋后再加樣。也可在板孔中加入稀釋液，再在其中加入血清標(biāo)本，然后在微型震蕩器上震蕩1分鐘以保證混和。加酶結(jié)合物應(yīng)用液和底物應(yīng)用液時可用定量多道加液器，使加液過程迅速完成。

人Kruppel樣因子2(KLF2)elisa測定 The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。

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